Review



representative clinical mrsa visa strain mu50  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    ATCC representative clinical mrsa visa strain mu50
    A Schematic overview of the CPPT setup for comparative analysis of VISA strain <t>Mu50</t> and a VSSA control. Cells were labelled with Vancomycin-BFL and PI. PI (+) -cells are depicted in gray, PI (–) -cells with different degrees of Vanco-BFL-labeling are indicated in light and dark green. PI (–) -cells were sorted onto Mueller-Hinton Agar (MHA) supplemented with different concentrations of vancomycin and colony formation was observed at different time points and for individual plates by time-lapse imaging. For VISA cells growth on a 0.25× MIC plate was used for cell fate determination (regular colony, delayed, smaller colony or no colony) prior to phenotypic backtracing of the ancestral phenotypic signatures in the flow cytometry dot plot. The exemplary dot plot shows all recorded Vanco-BFL ( +) , PI (–) events in light green, Vanco-BFL (+) , PI (+) -events in grey. The individually colored dots correspond to backtraced, sorted cells that were determined to belong the ´regular colony´ cell fate category. B Flow cytometry histogram showing the fluorescence signal in the Vanco-BFL-A channel of unstained and Vanco-BFL-labelled VSSA (blue) and VISA cells (red)´. C Percentage of sorted events that gave rise to colonies on agar plates with different concentrations of vancomycin. D Violin plot showing the cellular Vanco-BFL signal intensity of VISA cells for the different cell fate groups determined after growth on 0.25xMIC vancomycin. The graph shows the results of N = 960 sorted events from a single pre-sorting culture and is representative for 4 biological replicates. Statistical significance testing was performed by Kruskal–Wallis test in combination with Dunn´s multiple comparisons test. E Colony growth dynamics of sorted VISA cells in the presence (0.25xMIC) and absence of vancomycin as analyzed by time-lapse imaging analysis (compare Supplementary Movies , ). Growth ( Y -axis) was measured as contour size on the edge of the colony and is plotted against cultivation time. Raw data and calculations are available in Supplementary Data . The figure was created with BioRender.
    Representative Clinical Mrsa Visa Strain Mu50, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 814 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/representative clinical mrsa visa strain mu50/product/ATCC
    Average 99 stars, based on 814 article reviews
    representative clinical mrsa visa strain mu50 - by Bioz Stars, 2026-03
    99/100 stars

    Images

    1) Product Images from "Single-cell phenotypic profiling and backtracing exposes and predicts clinically relevant subpopulations in isogenic Staphylococcus aureus communities"

    Article Title: Single-cell phenotypic profiling and backtracing exposes and predicts clinically relevant subpopulations in isogenic Staphylococcus aureus communities

    Journal: Communications Biology

    doi: 10.1038/s42003-024-06894-z

    A Schematic overview of the CPPT setup for comparative analysis of VISA strain Mu50 and a VSSA control. Cells were labelled with Vancomycin-BFL and PI. PI (+) -cells are depicted in gray, PI (–) -cells with different degrees of Vanco-BFL-labeling are indicated in light and dark green. PI (–) -cells were sorted onto Mueller-Hinton Agar (MHA) supplemented with different concentrations of vancomycin and colony formation was observed at different time points and for individual plates by time-lapse imaging. For VISA cells growth on a 0.25× MIC plate was used for cell fate determination (regular colony, delayed, smaller colony or no colony) prior to phenotypic backtracing of the ancestral phenotypic signatures in the flow cytometry dot plot. The exemplary dot plot shows all recorded Vanco-BFL ( +) , PI (–) events in light green, Vanco-BFL (+) , PI (+) -events in grey. The individually colored dots correspond to backtraced, sorted cells that were determined to belong the ´regular colony´ cell fate category. B Flow cytometry histogram showing the fluorescence signal in the Vanco-BFL-A channel of unstained and Vanco-BFL-labelled VSSA (blue) and VISA cells (red)´. C Percentage of sorted events that gave rise to colonies on agar plates with different concentrations of vancomycin. D Violin plot showing the cellular Vanco-BFL signal intensity of VISA cells for the different cell fate groups determined after growth on 0.25xMIC vancomycin. The graph shows the results of N = 960 sorted events from a single pre-sorting culture and is representative for 4 biological replicates. Statistical significance testing was performed by Kruskal–Wallis test in combination with Dunn´s multiple comparisons test. E Colony growth dynamics of sorted VISA cells in the presence (0.25xMIC) and absence of vancomycin as analyzed by time-lapse imaging analysis (compare Supplementary Movies , ). Growth ( Y -axis) was measured as contour size on the edge of the colony and is plotted against cultivation time. Raw data and calculations are available in Supplementary Data . The figure was created with BioRender.
    Figure Legend Snippet: A Schematic overview of the CPPT setup for comparative analysis of VISA strain Mu50 and a VSSA control. Cells were labelled with Vancomycin-BFL and PI. PI (+) -cells are depicted in gray, PI (–) -cells with different degrees of Vanco-BFL-labeling are indicated in light and dark green. PI (–) -cells were sorted onto Mueller-Hinton Agar (MHA) supplemented with different concentrations of vancomycin and colony formation was observed at different time points and for individual plates by time-lapse imaging. For VISA cells growth on a 0.25× MIC plate was used for cell fate determination (regular colony, delayed, smaller colony or no colony) prior to phenotypic backtracing of the ancestral phenotypic signatures in the flow cytometry dot plot. The exemplary dot plot shows all recorded Vanco-BFL ( +) , PI (–) events in light green, Vanco-BFL (+) , PI (+) -events in grey. The individually colored dots correspond to backtraced, sorted cells that were determined to belong the ´regular colony´ cell fate category. B Flow cytometry histogram showing the fluorescence signal in the Vanco-BFL-A channel of unstained and Vanco-BFL-labelled VSSA (blue) and VISA cells (red)´. C Percentage of sorted events that gave rise to colonies on agar plates with different concentrations of vancomycin. D Violin plot showing the cellular Vanco-BFL signal intensity of VISA cells for the different cell fate groups determined after growth on 0.25xMIC vancomycin. The graph shows the results of N = 960 sorted events from a single pre-sorting culture and is representative for 4 biological replicates. Statistical significance testing was performed by Kruskal–Wallis test in combination with Dunn´s multiple comparisons test. E Colony growth dynamics of sorted VISA cells in the presence (0.25xMIC) and absence of vancomycin as analyzed by time-lapse imaging analysis (compare Supplementary Movies , ). Growth ( Y -axis) was measured as contour size on the edge of the colony and is plotted against cultivation time. Raw data and calculations are available in Supplementary Data . The figure was created with BioRender.

    Techniques Used: Control, Labeling, Imaging, Flow Cytometry, Fluorescence

    Bacterial strains used in this study
    Figure Legend Snippet: Bacterial strains used in this study

    Techniques Used: Isolation, Plasmid Preparation



    Similar Products

    representative clinical mrsa visa strain mu50  (ATCC)
    99
    ATCC representative clinical mrsa visa strain mu50
    A Schematic overview of the CPPT setup for comparative analysis of VISA strain <t>Mu50</t> and a VSSA control. Cells were labelled with Vancomycin-BFL and PI. PI (+) -cells are depicted in gray, PI (–) -cells with different degrees of Vanco-BFL-labeling are indicated in light and dark green. PI (–) -cells were sorted onto Mueller-Hinton Agar (MHA) supplemented with different concentrations of vancomycin and colony formation was observed at different time points and for individual plates by time-lapse imaging. For VISA cells growth on a 0.25× MIC plate was used for cell fate determination (regular colony, delayed, smaller colony or no colony) prior to phenotypic backtracing of the ancestral phenotypic signatures in the flow cytometry dot plot. The exemplary dot plot shows all recorded Vanco-BFL ( +) , PI (–) events in light green, Vanco-BFL (+) , PI (+) -events in grey. The individually colored dots correspond to backtraced, sorted cells that were determined to belong the ´regular colony´ cell fate category. B Flow cytometry histogram showing the fluorescence signal in the Vanco-BFL-A channel of unstained and Vanco-BFL-labelled VSSA (blue) and VISA cells (red)´. C Percentage of sorted events that gave rise to colonies on agar plates with different concentrations of vancomycin. D Violin plot showing the cellular Vanco-BFL signal intensity of VISA cells for the different cell fate groups determined after growth on 0.25xMIC vancomycin. The graph shows the results of N = 960 sorted events from a single pre-sorting culture and is representative for 4 biological replicates. Statistical significance testing was performed by Kruskal–Wallis test in combination with Dunn´s multiple comparisons test. E Colony growth dynamics of sorted VISA cells in the presence (0.25xMIC) and absence of vancomycin as analyzed by time-lapse imaging analysis (compare Supplementary Movies , ). Growth ( Y -axis) was measured as contour size on the edge of the colony and is plotted against cultivation time. Raw data and calculations are available in Supplementary Data . The figure was created with BioRender.
    Representative Clinical Mrsa Visa Strain Mu50, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/representative clinical mrsa visa strain mu50/product/ATCC
    Average 99 stars, based on 1 article reviews
    representative clinical mrsa visa strain mu50 - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    china staphylococcus aureus atcc 29213 control strain atcc staphylococcus aureus ci mrsa clinical  (ATCC)
    99
    ATCC china staphylococcus aureus atcc 29213 control strain atcc staphylococcus aureus ci mrsa clinical
    A Schematic overview of the CPPT setup for comparative analysis of VISA strain <t>Mu50</t> and a VSSA control. Cells were labelled with Vancomycin-BFL and PI. PI (+) -cells are depicted in gray, PI (–) -cells with different degrees of Vanco-BFL-labeling are indicated in light and dark green. PI (–) -cells were sorted onto Mueller-Hinton Agar (MHA) supplemented with different concentrations of vancomycin and colony formation was observed at different time points and for individual plates by time-lapse imaging. For VISA cells growth on a 0.25× MIC plate was used for cell fate determination (regular colony, delayed, smaller colony or no colony) prior to phenotypic backtracing of the ancestral phenotypic signatures in the flow cytometry dot plot. The exemplary dot plot shows all recorded Vanco-BFL ( +) , PI (–) events in light green, Vanco-BFL (+) , PI (+) -events in grey. The individually colored dots correspond to backtraced, sorted cells that were determined to belong the ´regular colony´ cell fate category. B Flow cytometry histogram showing the fluorescence signal in the Vanco-BFL-A channel of unstained and Vanco-BFL-labelled VSSA (blue) and VISA cells (red)´. C Percentage of sorted events that gave rise to colonies on agar plates with different concentrations of vancomycin. D Violin plot showing the cellular Vanco-BFL signal intensity of VISA cells for the different cell fate groups determined after growth on 0.25xMIC vancomycin. The graph shows the results of N = 960 sorted events from a single pre-sorting culture and is representative for 4 biological replicates. Statistical significance testing was performed by Kruskal–Wallis test in combination with Dunn´s multiple comparisons test. E Colony growth dynamics of sorted VISA cells in the presence (0.25xMIC) and absence of vancomycin as analyzed by time-lapse imaging analysis (compare Supplementary Movies , ). Growth ( Y -axis) was measured as contour size on the edge of the colony and is plotted against cultivation time. Raw data and calculations are available in Supplementary Data . The figure was created with BioRender.
    China Staphylococcus Aureus Atcc 29213 Control Strain Atcc Staphylococcus Aureus Ci Mrsa Clinical, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/china staphylococcus aureus atcc 29213 control strain atcc staphylococcus aureus ci mrsa clinical/product/ATCC
    Average 99 stars, based on 1 article reviews
    china staphylococcus aureus atcc 29213 control strain atcc staphylococcus aureus ci mrsa clinical - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    clinical mrsa strains  (ATCC)
    98
    ATCC clinical mrsa strains
    A Schematic overview of the CPPT setup for comparative analysis of VISA strain <t>Mu50</t> and a VSSA control. Cells were labelled with Vancomycin-BFL and PI. PI (+) -cells are depicted in gray, PI (–) -cells with different degrees of Vanco-BFL-labeling are indicated in light and dark green. PI (–) -cells were sorted onto Mueller-Hinton Agar (MHA) supplemented with different concentrations of vancomycin and colony formation was observed at different time points and for individual plates by time-lapse imaging. For VISA cells growth on a 0.25× MIC plate was used for cell fate determination (regular colony, delayed, smaller colony or no colony) prior to phenotypic backtracing of the ancestral phenotypic signatures in the flow cytometry dot plot. The exemplary dot plot shows all recorded Vanco-BFL ( +) , PI (–) events in light green, Vanco-BFL (+) , PI (+) -events in grey. The individually colored dots correspond to backtraced, sorted cells that were determined to belong the ´regular colony´ cell fate category. B Flow cytometry histogram showing the fluorescence signal in the Vanco-BFL-A channel of unstained and Vanco-BFL-labelled VSSA (blue) and VISA cells (red)´. C Percentage of sorted events that gave rise to colonies on agar plates with different concentrations of vancomycin. D Violin plot showing the cellular Vanco-BFL signal intensity of VISA cells for the different cell fate groups determined after growth on 0.25xMIC vancomycin. The graph shows the results of N = 960 sorted events from a single pre-sorting culture and is representative for 4 biological replicates. Statistical significance testing was performed by Kruskal–Wallis test in combination with Dunn´s multiple comparisons test. E Colony growth dynamics of sorted VISA cells in the presence (0.25xMIC) and absence of vancomycin as analyzed by time-lapse imaging analysis (compare Supplementary Movies , ). Growth ( Y -axis) was measured as contour size on the edge of the colony and is plotted against cultivation time. Raw data and calculations are available in Supplementary Data . The figure was created with BioRender.
    Clinical Mrsa Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clinical mrsa strains/product/ATCC
    Average 98 stars, based on 1 article reviews
    clinical mrsa strains - by Bioz Stars, 2026-03
    98/100 stars
    		  Buy from Supplier
    mrsa laboratory strain atcc 25923 clinical isolate mrsa laboratory strain mrsa 15 st39  (ATCC)
    99
    ATCC mrsa laboratory strain atcc 25923 clinical isolate mrsa laboratory strain mrsa 15 st39
    A Schematic overview of the CPPT setup for comparative analysis of VISA strain <t>Mu50</t> and a VSSA control. Cells were labelled with Vancomycin-BFL and PI. PI (+) -cells are depicted in gray, PI (–) -cells with different degrees of Vanco-BFL-labeling are indicated in light and dark green. PI (–) -cells were sorted onto Mueller-Hinton Agar (MHA) supplemented with different concentrations of vancomycin and colony formation was observed at different time points and for individual plates by time-lapse imaging. For VISA cells growth on a 0.25× MIC plate was used for cell fate determination (regular colony, delayed, smaller colony or no colony) prior to phenotypic backtracing of the ancestral phenotypic signatures in the flow cytometry dot plot. The exemplary dot plot shows all recorded Vanco-BFL ( +) , PI (–) events in light green, Vanco-BFL (+) , PI (+) -events in grey. The individually colored dots correspond to backtraced, sorted cells that were determined to belong the ´regular colony´ cell fate category. B Flow cytometry histogram showing the fluorescence signal in the Vanco-BFL-A channel of unstained and Vanco-BFL-labelled VSSA (blue) and VISA cells (red)´. C Percentage of sorted events that gave rise to colonies on agar plates with different concentrations of vancomycin. D Violin plot showing the cellular Vanco-BFL signal intensity of VISA cells for the different cell fate groups determined after growth on 0.25xMIC vancomycin. The graph shows the results of N = 960 sorted events from a single pre-sorting culture and is representative for 4 biological replicates. Statistical significance testing was performed by Kruskal–Wallis test in combination with Dunn´s multiple comparisons test. E Colony growth dynamics of sorted VISA cells in the presence (0.25xMIC) and absence of vancomycin as analyzed by time-lapse imaging analysis (compare Supplementary Movies , ). Growth ( Y -axis) was measured as contour size on the edge of the colony and is plotted against cultivation time. Raw data and calculations are available in Supplementary Data . The figure was created with BioRender.
    Mrsa Laboratory Strain Atcc 25923 Clinical Isolate Mrsa Laboratory Strain Mrsa 15 St39, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrsa laboratory strain atcc 25923 clinical isolate mrsa laboratory strain mrsa 15 st39/product/ATCC
    Average 99 stars, based on 1 article reviews
    mrsa laboratory strain atcc 25923 clinical isolate mrsa laboratory strain mrsa 15 st39 - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    clinical mrsa strain  (ATCC)
    94
    ATCC clinical mrsa strain
    A Schematic overview of the CPPT setup for comparative analysis of VISA strain <t>Mu50</t> and a VSSA control. Cells were labelled with Vancomycin-BFL and PI. PI (+) -cells are depicted in gray, PI (–) -cells with different degrees of Vanco-BFL-labeling are indicated in light and dark green. PI (–) -cells were sorted onto Mueller-Hinton Agar (MHA) supplemented with different concentrations of vancomycin and colony formation was observed at different time points and for individual plates by time-lapse imaging. For VISA cells growth on a 0.25× MIC plate was used for cell fate determination (regular colony, delayed, smaller colony or no colony) prior to phenotypic backtracing of the ancestral phenotypic signatures in the flow cytometry dot plot. The exemplary dot plot shows all recorded Vanco-BFL ( +) , PI (–) events in light green, Vanco-BFL (+) , PI (+) -events in grey. The individually colored dots correspond to backtraced, sorted cells that were determined to belong the ´regular colony´ cell fate category. B Flow cytometry histogram showing the fluorescence signal in the Vanco-BFL-A channel of unstained and Vanco-BFL-labelled VSSA (blue) and VISA cells (red)´. C Percentage of sorted events that gave rise to colonies on agar plates with different concentrations of vancomycin. D Violin plot showing the cellular Vanco-BFL signal intensity of VISA cells for the different cell fate groups determined after growth on 0.25xMIC vancomycin. The graph shows the results of N = 960 sorted events from a single pre-sorting culture and is representative for 4 biological replicates. Statistical significance testing was performed by Kruskal–Wallis test in combination with Dunn´s multiple comparisons test. E Colony growth dynamics of sorted VISA cells in the presence (0.25xMIC) and absence of vancomycin as analyzed by time-lapse imaging analysis (compare Supplementary Movies , ). Growth ( Y -axis) was measured as contour size on the edge of the colony and is plotted against cultivation time. Raw data and calculations are available in Supplementary Data . The figure was created with BioRender.
    Clinical Mrsa Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clinical mrsa strain/product/ATCC
    Average 94 stars, based on 1 article reviews
    clinical mrsa strain - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    vre clinical strain d mrsa atcc 43300  (ATCC)
    99
    ATCC vre clinical strain d mrsa atcc 43300
    A Schematic overview of the CPPT setup for comparative analysis of VISA strain <t>Mu50</t> and a VSSA control. Cells were labelled with Vancomycin-BFL and PI. PI (+) -cells are depicted in gray, PI (–) -cells with different degrees of Vanco-BFL-labeling are indicated in light and dark green. PI (–) -cells were sorted onto Mueller-Hinton Agar (MHA) supplemented with different concentrations of vancomycin and colony formation was observed at different time points and for individual plates by time-lapse imaging. For VISA cells growth on a 0.25× MIC plate was used for cell fate determination (regular colony, delayed, smaller colony or no colony) prior to phenotypic backtracing of the ancestral phenotypic signatures in the flow cytometry dot plot. The exemplary dot plot shows all recorded Vanco-BFL ( +) , PI (–) events in light green, Vanco-BFL (+) , PI (+) -events in grey. The individually colored dots correspond to backtraced, sorted cells that were determined to belong the ´regular colony´ cell fate category. B Flow cytometry histogram showing the fluorescence signal in the Vanco-BFL-A channel of unstained and Vanco-BFL-labelled VSSA (blue) and VISA cells (red)´. C Percentage of sorted events that gave rise to colonies on agar plates with different concentrations of vancomycin. D Violin plot showing the cellular Vanco-BFL signal intensity of VISA cells for the different cell fate groups determined after growth on 0.25xMIC vancomycin. The graph shows the results of N = 960 sorted events from a single pre-sorting culture and is representative for 4 biological replicates. Statistical significance testing was performed by Kruskal–Wallis test in combination with Dunn´s multiple comparisons test. E Colony growth dynamics of sorted VISA cells in the presence (0.25xMIC) and absence of vancomycin as analyzed by time-lapse imaging analysis (compare Supplementary Movies , ). Growth ( Y -axis) was measured as contour size on the edge of the colony and is plotted against cultivation time. Raw data and calculations are available in Supplementary Data . The figure was created with BioRender.
    Vre Clinical Strain D Mrsa Atcc 43300, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vre clinical strain d mrsa atcc 43300/product/ATCC
    Average 99 stars, based on 1 article reviews
    vre clinical strain d mrsa atcc 43300 - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    2017 mrsa clinical strain mssa atcc 29213 e coli atcc 25922  (ATCC)
    99
    ATCC 2017 mrsa clinical strain mssa atcc 29213 e coli atcc 25922
    A Schematic overview of the CPPT setup for comparative analysis of VISA strain <t>Mu50</t> and a VSSA control. Cells were labelled with Vancomycin-BFL and PI. PI (+) -cells are depicted in gray, PI (–) -cells with different degrees of Vanco-BFL-labeling are indicated in light and dark green. PI (–) -cells were sorted onto Mueller-Hinton Agar (MHA) supplemented with different concentrations of vancomycin and colony formation was observed at different time points and for individual plates by time-lapse imaging. For VISA cells growth on a 0.25× MIC plate was used for cell fate determination (regular colony, delayed, smaller colony or no colony) prior to phenotypic backtracing of the ancestral phenotypic signatures in the flow cytometry dot plot. The exemplary dot plot shows all recorded Vanco-BFL ( +) , PI (–) events in light green, Vanco-BFL (+) , PI (+) -events in grey. The individually colored dots correspond to backtraced, sorted cells that were determined to belong the ´regular colony´ cell fate category. B Flow cytometry histogram showing the fluorescence signal in the Vanco-BFL-A channel of unstained and Vanco-BFL-labelled VSSA (blue) and VISA cells (red)´. C Percentage of sorted events that gave rise to colonies on agar plates with different concentrations of vancomycin. D Violin plot showing the cellular Vanco-BFL signal intensity of VISA cells for the different cell fate groups determined after growth on 0.25xMIC vancomycin. The graph shows the results of N = 960 sorted events from a single pre-sorting culture and is representative for 4 biological replicates. Statistical significance testing was performed by Kruskal–Wallis test in combination with Dunn´s multiple comparisons test. E Colony growth dynamics of sorted VISA cells in the presence (0.25xMIC) and absence of vancomycin as analyzed by time-lapse imaging analysis (compare Supplementary Movies , ). Growth ( Y -axis) was measured as contour size on the edge of the colony and is plotted against cultivation time. Raw data and calculations are available in Supplementary Data . The figure was created with BioRender.
    2017 Mrsa Clinical Strain Mssa Atcc 29213 E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2017 mrsa clinical strain mssa atcc 29213 e coli atcc 25922/product/ATCC
    Average 99 stars, based on 1 article reviews
    2017 mrsa clinical strain mssa atcc 29213 e coli atcc 25922 - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    mrsa clinical strain mssa atcc 29213 e coli atcc 25922  (ATCC)
    99
    ATCC mrsa clinical strain mssa atcc 29213 e coli atcc 25922
    A Schematic overview of the CPPT setup for comparative analysis of VISA strain <t>Mu50</t> and a VSSA control. Cells were labelled with Vancomycin-BFL and PI. PI (+) -cells are depicted in gray, PI (–) -cells with different degrees of Vanco-BFL-labeling are indicated in light and dark green. PI (–) -cells were sorted onto Mueller-Hinton Agar (MHA) supplemented with different concentrations of vancomycin and colony formation was observed at different time points and for individual plates by time-lapse imaging. For VISA cells growth on a 0.25× MIC plate was used for cell fate determination (regular colony, delayed, smaller colony or no colony) prior to phenotypic backtracing of the ancestral phenotypic signatures in the flow cytometry dot plot. The exemplary dot plot shows all recorded Vanco-BFL ( +) , PI (–) events in light green, Vanco-BFL (+) , PI (+) -events in grey. The individually colored dots correspond to backtraced, sorted cells that were determined to belong the ´regular colony´ cell fate category. B Flow cytometry histogram showing the fluorescence signal in the Vanco-BFL-A channel of unstained and Vanco-BFL-labelled VSSA (blue) and VISA cells (red)´. C Percentage of sorted events that gave rise to colonies on agar plates with different concentrations of vancomycin. D Violin plot showing the cellular Vanco-BFL signal intensity of VISA cells for the different cell fate groups determined after growth on 0.25xMIC vancomycin. The graph shows the results of N = 960 sorted events from a single pre-sorting culture and is representative for 4 biological replicates. Statistical significance testing was performed by Kruskal–Wallis test in combination with Dunn´s multiple comparisons test. E Colony growth dynamics of sorted VISA cells in the presence (0.25xMIC) and absence of vancomycin as analyzed by time-lapse imaging analysis (compare Supplementary Movies , ). Growth ( Y -axis) was measured as contour size on the edge of the colony and is plotted against cultivation time. Raw data and calculations are available in Supplementary Data . The figure was created with BioRender.
    Mrsa Clinical Strain Mssa Atcc 29213 E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrsa clinical strain mssa atcc 29213 e coli atcc 25922/product/ATCC
    Average 99 stars, based on 1 article reviews
    mrsa clinical strain mssa atcc 29213 e coli atcc 25922 - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    clinical isolated mrsa strains  (ATCC)
    99
    ATCC clinical isolated mrsa strains
    A Schematic overview of the CPPT setup for comparative analysis of VISA strain <t>Mu50</t> and a VSSA control. Cells were labelled with Vancomycin-BFL and PI. PI (+) -cells are depicted in gray, PI (–) -cells with different degrees of Vanco-BFL-labeling are indicated in light and dark green. PI (–) -cells were sorted onto Mueller-Hinton Agar (MHA) supplemented with different concentrations of vancomycin and colony formation was observed at different time points and for individual plates by time-lapse imaging. For VISA cells growth on a 0.25× MIC plate was used for cell fate determination (regular colony, delayed, smaller colony or no colony) prior to phenotypic backtracing of the ancestral phenotypic signatures in the flow cytometry dot plot. The exemplary dot plot shows all recorded Vanco-BFL ( +) , PI (–) events in light green, Vanco-BFL (+) , PI (+) -events in grey. The individually colored dots correspond to backtraced, sorted cells that were determined to belong the ´regular colony´ cell fate category. B Flow cytometry histogram showing the fluorescence signal in the Vanco-BFL-A channel of unstained and Vanco-BFL-labelled VSSA (blue) and VISA cells (red)´. C Percentage of sorted events that gave rise to colonies on agar plates with different concentrations of vancomycin. D Violin plot showing the cellular Vanco-BFL signal intensity of VISA cells for the different cell fate groups determined after growth on 0.25xMIC vancomycin. The graph shows the results of N = 960 sorted events from a single pre-sorting culture and is representative for 4 biological replicates. Statistical significance testing was performed by Kruskal–Wallis test in combination with Dunn´s multiple comparisons test. E Colony growth dynamics of sorted VISA cells in the presence (0.25xMIC) and absence of vancomycin as analyzed by time-lapse imaging analysis (compare Supplementary Movies , ). Growth ( Y -axis) was measured as contour size on the edge of the colony and is plotted against cultivation time. Raw data and calculations are available in Supplementary Data . The figure was created with BioRender.
    Clinical Isolated Mrsa Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clinical isolated mrsa strains/product/ATCC
    Average 99 stars, based on 1 article reviews
    clinical isolated mrsa strains - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    A Schematic overview of the CPPT setup for comparative analysis of VISA strain Mu50 and a VSSA control. Cells were labelled with Vancomycin-BFL and PI. PI (+) -cells are depicted in gray, PI (–) -cells with different degrees of Vanco-BFL-labeling are indicated in light and dark green. PI (–) -cells were sorted onto Mueller-Hinton Agar (MHA) supplemented with different concentrations of vancomycin and colony formation was observed at different time points and for individual plates by time-lapse imaging. For VISA cells growth on a 0.25× MIC plate was used for cell fate determination (regular colony, delayed, smaller colony or no colony) prior to phenotypic backtracing of the ancestral phenotypic signatures in the flow cytometry dot plot. The exemplary dot plot shows all recorded Vanco-BFL ( +) , PI (–) events in light green, Vanco-BFL (+) , PI (+) -events in grey. The individually colored dots correspond to backtraced, sorted cells that were determined to belong the ´regular colony´ cell fate category. B Flow cytometry histogram showing the fluorescence signal in the Vanco-BFL-A channel of unstained and Vanco-BFL-labelled VSSA (blue) and VISA cells (red)´. C Percentage of sorted events that gave rise to colonies on agar plates with different concentrations of vancomycin. D Violin plot showing the cellular Vanco-BFL signal intensity of VISA cells for the different cell fate groups determined after growth on 0.25xMIC vancomycin. The graph shows the results of N = 960 sorted events from a single pre-sorting culture and is representative for 4 biological replicates. Statistical significance testing was performed by Kruskal–Wallis test in combination with Dunn´s multiple comparisons test. E Colony growth dynamics of sorted VISA cells in the presence (0.25xMIC) and absence of vancomycin as analyzed by time-lapse imaging analysis (compare Supplementary Movies , ). Growth ( Y -axis) was measured as contour size on the edge of the colony and is plotted against cultivation time. Raw data and calculations are available in Supplementary Data . The figure was created with BioRender.

    Journal: Communications Biology

    Article Title: Single-cell phenotypic profiling and backtracing exposes and predicts clinically relevant subpopulations in isogenic Staphylococcus aureus communities

    doi: 10.1038/s42003-024-06894-z

    Figure Lengend Snippet: A Schematic overview of the CPPT setup for comparative analysis of VISA strain Mu50 and a VSSA control. Cells were labelled with Vancomycin-BFL and PI. PI (+) -cells are depicted in gray, PI (–) -cells with different degrees of Vanco-BFL-labeling are indicated in light and dark green. PI (–) -cells were sorted onto Mueller-Hinton Agar (MHA) supplemented with different concentrations of vancomycin and colony formation was observed at different time points and for individual plates by time-lapse imaging. For VISA cells growth on a 0.25× MIC plate was used for cell fate determination (regular colony, delayed, smaller colony or no colony) prior to phenotypic backtracing of the ancestral phenotypic signatures in the flow cytometry dot plot. The exemplary dot plot shows all recorded Vanco-BFL ( +) , PI (–) events in light green, Vanco-BFL (+) , PI (+) -events in grey. The individually colored dots correspond to backtraced, sorted cells that were determined to belong the ´regular colony´ cell fate category. B Flow cytometry histogram showing the fluorescence signal in the Vanco-BFL-A channel of unstained and Vanco-BFL-labelled VSSA (blue) and VISA cells (red)´. C Percentage of sorted events that gave rise to colonies on agar plates with different concentrations of vancomycin. D Violin plot showing the cellular Vanco-BFL signal intensity of VISA cells for the different cell fate groups determined after growth on 0.25xMIC vancomycin. The graph shows the results of N = 960 sorted events from a single pre-sorting culture and is representative for 4 biological replicates. Statistical significance testing was performed by Kruskal–Wallis test in combination with Dunn´s multiple comparisons test. E Colony growth dynamics of sorted VISA cells in the presence (0.25xMIC) and absence of vancomycin as analyzed by time-lapse imaging analysis (compare Supplementary Movies , ). Growth ( Y -axis) was measured as contour size on the edge of the colony and is plotted against cultivation time. Raw data and calculations are available in Supplementary Data . The figure was created with BioRender.

    Article Snippet: Single colonies (3 biological replicates) of the vancomycin susceptible S. aureus strain ATCC 29213 and representative clinical MRSA-VISA strain Mu50 (ATCC 700699) were grown overnight in TSB at 37 °C.

    Techniques: Control, Labeling, Imaging, Flow Cytometry, Fluorescence

    Bacterial strains used in this study

    Journal: Communications Biology

    Article Title: Single-cell phenotypic profiling and backtracing exposes and predicts clinically relevant subpopulations in isogenic Staphylococcus aureus communities

    doi: 10.1038/s42003-024-06894-z

    Figure Lengend Snippet: Bacterial strains used in this study

    Article Snippet: Single colonies (3 biological replicates) of the vancomycin susceptible S. aureus strain ATCC 29213 and representative clinical MRSA-VISA strain Mu50 (ATCC 700699) were grown overnight in TSB at 37 °C.

    Techniques: Isolation, Plasmid Preparation